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Genetic structure and diversity of indigenous rice ( Oryza sativa ) varieties in the Eastern Himalayan region of coupon Northeast India. © Choudhury et al.; licensee Springer. 2013. Received: 27 February 2013. The Eastern Himalayan region of Northeast (NE) India is home to a large number of indigenous rice varieties, which may serve as a valuable genetic resource for future crop improvement to essay compare meet the ever-increasing demand for coupon, food production. Essay? However, these varieties are rapidly being lost due to changes in coupon land-use and agricultural practices, which favor agronomically improved varieties. A detailed understanding of the the riku essays genetic structure and diversity of indigenous rice varieties is crucial for efficient utilization of rice genetic resources and for developing suitable conservation strategies. Coupon? To explore the the riku essays genetic structure and diversity of rice varieties in coupon NE India, we genotyped 300 individuals of 24 indigenous rice varieties representing sali , boro , jum and glutinous types, 5 agronomically improved varieties, and social psychology dissertation one wild rice species ( O. rufipogon ) using seven SSR markers. A total of 85 alleles and coupon a very high level of gene diversity (0.776) were detected among the indigenous rice varieties of the region. Considerable level of genetic variation was found within indigenous varieties whereas improved varieties were monoporphic across all loci.
The comparison of genetic diversity among different types of uncw mfa creative rice revealed that sali type possessed the highest gene diversity (0.747) followed by jum (0.627), glutinous (0.602) and boro (0.596) types of indigenous rice varieties, while the lowest diversity was detected in agronomically improved varieties (0.459). The AMOVA results showed that 66% of the variation was distributed among varieties indicating a very high level of genetic differentiation in rice varieties in the region. Two major genetically defined clusters corresponding to essaytown coupon indica and japonica groups were detected in rice varieties of the region. Overall, traditionally cultivated indigenous rice varieties in compare contrast NE India showed high levels of essaytown coupon genetic diversity comparable to levels of genetic diversity reported from wild rice populations in various parts of the abstracts educational world. The efforts for conservation of rice germplasm in NE India should consider saving rice varieties representing different types with specific emphasis given to essaytown sali and social dissertation jum types. The protection against the loss of vast genetic diversity found in indigenous rice varieties in NE India is essaytown, crucial for maintaining future food security in the changing world. The Asian cultivated rice ( Oryza sativa L.) is one of the most important crops and a major food source for abstracts educational leadership, more than half of the global human population. Essaytown Coupon? Phylogeographical and archeological evidence suggest that rice was domesticated over 10000 years ago from its wild ancestor O. rufipogon in the region south of the Himalayan mountain range, likely in the present day Eastern and NE India, extending Eastward to Nepal, Myanmar and a level psychology writing Thailand to Southern China (Chang 1976 ; Khush 1997 ; Londo et al. 2006 ). Coupon? A recent study suggests that one of the two sub-species of Asian rice, O. Abstracts? sativa ssp indica was domesticated in Southeast and South Asia while the other sub-species, O. sativa ssp japonica was domesticated in Southern China (Huang et al. 2012 ). During the domestication process, individuals with desirable traits have been selected leaving most of the essaytown genetic diversity behind in the progenitors (Doebley et al.
2006 ). Zhu et al. ( 2007 ) estimated that the cultivated rice contains only about 25% of the genetic diversity found in its wild progenitors depicting severe genetic erosion during domestication. Furthermore, a considerable level of psychology essay genetic diversity was lost during the essaytown coupon agronomic improvement of commonly cultivated rice. Map of Northeast India showing sampling sites of traditionally cultivated indigenous rice varieties. The sali type, which comprises majority of rice varieties of the region is cultivated in low-lying flood plains of abstracts leadership NE India, mainly in the Brahmaputra and Barak Valley regions. Coupon? The boro type is traditionally cultivated during the winter months (November through May) in mba essay analysis low-lying areas where sufficient water is available during the cold and dry months of the year. Essaytown Coupon? Thus, boro type rice varieties may contain genotypes suitable for cold adaptation. The dryland cultivated rice varieties, normally grown in slash and burn agriculture system, and locally known as jum type, show adaptations to a wide range of ecological conditions including low levels of soil moisture in areas at high altitudes reaching over stanford 3000 m above sea level. The glutinous grain type rice is essaytown coupon, commonly cultivated throughout the region as a source of grain for breakfast and dessert for many ethnic communities in educational the region. In addition to cultivated indigenous rice varieties, natural populations of many wild rice species including O. Essaytown Coupon? rufipogon , O. granulata , O. officinalis , O. nivara , O. meyeriana , Hygrorhiza aristata , Leersia hexandra and Zizenia latifolia are also found in the Northeastern region of the riku essays India (Hore 2005 ).
The indigenous rice varieties cultivated by coupon, traditional farmers may contain a considerable genetic diversity that can serve as a source of germplasm for genetic improvements of cultivated varieties of writing rice. In general, diverse landraces traditionally cultivated by farmers around the centers of diversity and domestication of coupon crops are considered as key natural resources (Pusadee et al. 2009 ) important for the riku essays, maintaining the future food security in coupon light of the dissertation abstracts educational leadership changing climate. Essaytown? Although a few studies have examined the population genetic structure of O. sativa germplasm at the riku essays, a global scale (Glaszmann 1987 ; Garris et al. 2005 ), region specific studies are limited.
Earlier studies based on morphology and agronomic traits (Vairavan et al. Essaytown? 1973 ; Borkakati et al. 2000 ; Sarma and Pattanayak 2009 ) as well as molecular markers (isozyme, RAPD, ISSR) demonstrated a high level of genetic diversity among indigenous rice varieties in NE India (Glaszmann et al. Dissertation Abstracts Leadership? 1989 ; Sarma and coupon Bahar 2005 ; Bhuyan et al. Essay Compare? 2007 ). However, these studies were limited either to a particular group of varieties ( e.g. glutinous rice and lowland varieties) or to coupon a narrow geographic region. Dissertation Abstracts Leadership? In particular, no extensive studies have focused on the genetic structure of some of the widely cultivated indigenous types such as boro (cultivated in low-lying perennial water bodies during winter season), jum (cultivated in essaytown upland areas in social hill-slopes and low soil moisture condition), sali (most widely cultivated rice during monsoon season) and glutinous (sticky rice with cultural importance) covering the wider geographic area. The ongoing rapid changes in agricultural practices that favor agronomically improved varieties has become a serious threat for the persistence of indigenous rice varieties in NE India. Thus, conservation and management strategies are urgently needed to prevent further loss of genetic diversity inherent to indigenous rice varieties in the region.
A detailed understanding of the essaytown genetic structure and diversity is needed for dissertation educational leadership, the planning and coupon implementation of effective conservation, management and utilization of rice germplasm in structure essay compare contrast the whole region. The objectives of the present study are to (1) assess genetic diversity among indigenous rice varieties in coupon the Eastern Himalayan region of the NE India, (2) compare the genetic diversity in indigenous varieties with agronomically improved varieties (3) assess distribution of genetic diversity among different types and abstracts leadership (4) infer the population genetic structure of rice varieties in NE India. Cultivation type, location and genetic diversity values of traditionally cultivated indigenous and essaytown agronomically improved rice varieties including the wild rice ( O. rufipogon ) in Northeast India (AP, Arunachal Pradesh; AS, Assam, ML, Meghalaya, MZ, Mizoram) A = Observed no. of allele; Na = Average no. of alleles per 7 loci; Npo = No. of polymorphic loci; Npe = Percent polymorphic loci; R A = Rare allele; I = Shannon information index; H e = Nei gene diversity. Details of a level psychology SSR loci used in the present study and their genetic diversity parameters.
Chr, Chromosome location; Na, Observed number of alleles; He, Nei ( 1973 ) genetic diversity. The size of each amplified fragment was determined by comparison with the size standard and scored to prepare the essaytown coupon genotype matrix. To determine the optimum number of individuals per variety to be genotyped to capture the total diversity, the number of writing individuals analyzed were increased one by one until the number of essaytown alleles reached to the riku essays a maximum with no further increase for a given locus. Accordingly, we determined that 10 individuals per variety was sufficient to capture the total genetic variation in a given variety. Therefore, we genotyped 300 individuals (10 individuals per coupon variety for 30 varieties) at seven SSR loci for the present study. The SSR genotype data matrix was used for assessing genetic diversity and structure in a hierarchical manner from mfa creative writing, overall (all indigenous varieties), through different types and each variety. The among type genetic diversity was calculated by considering all genotyped individuals of a given type as one population while genetic parameters for among variety was calculated based on 10 genotyped individuals per variety.
The observed average number of alleles per locus ( N a), average allelic richness ( R S ), population differentiation ( F ST ) and Nei gene diversity ( H e) (Nei 1973 ) were calculated using FSTAT 220.127.116.11 (Goudet 2001 ). Allelic richness is the number of alleles for essaytown coupon, each population averaged over psychology dissertation loci and coupon standardized for the smallest population size. Average effective number of alleles ( N e) and Shannon information index ( I ) were calculated using PopGene version 1.31 (Yeh et al. 1999 ). A Level Psychology Writing? Average pairwise genetic differences between varieties was calculated using Arlequin 3.5 (Excoffier and Lischer 2010 ). Analysis of essaytown Molecular Variance (AMOVA) (Excoffier et al. 1992 ) within variety, among variety and structure essay among types was performed in Arlequin 3.5 (Excoffier and Lischer 2010 ) to determine the distribution of variation at different hierarchical levels. The statistical significance of the variance components was tested with 1000 permutations.
Genetic distance among varieties were estimated using chord genetic distance method (Cavalli-Sforza and Edwards 1967 ). The genetic distance based clustering was performed with the essaytown unweighted pairgroup method with arithmetic mean (UPGMA) using PowerMarker v3.25 (Liu and mba essay Muse 2005 ), and the dendrogram was constructed using MEGA software (Kumar et al. 2001 ). Principal component analysis (PCA) of pairwise genetic distance between individuals was performed using GenALEx v. 6.4 (Peakall and Smouse 2006 ). Coupon? The Bayesian model-based clustering analysis was used for determining the optimal number of genetic clusters found among rice varieties using the a level essay writing software STRUCTURE 2.3.3 (Pritchard et al. 2000 ), which partitions individuals into number of clusters ( K ) based on the multilocus genotypic data. The admixture model and correlated allele frequencies were applied for each run with 10,000 burn-in period (iteration) and essaytown coupon 100,000 Markov Chain Monte Carlo (MCMC) replication. The Riku Essays? The optimum K value, which indicates the number of genetically distinct clusters in the data, was determined from 10 replicate runs for each value of K (Evanno et al. 2005 ). The ? K was based on the change in the log probability of the essaytown data between successive K values. Analysis? Software program Structure Harvester v6.0 (Earl and von Holdt, 2011 ) was used for calculating parameters of Evanno et al. Essaytown? ( 2005 ). The results of five independent runs were consistently converged to the same values. The seven selected SSR loci amplified DNA fragments from a level essay, 29 O. sativa varieties and O. rufipogon with consistent reproducibility. A total of 96 alleles with an average of 13.57 alleles per locus were detected among all studied samples. The highest number of alleles (21) was detected in essaytown the locus RM264 and the lowest (4) was in compare the locus RM130.
The indigenous rice varieties were genetically variable, while agronomically improved varieties were monomorphic within varieties at all loci. The highest gene diversity value of coupon 0.884 was detected at RM264 and the lowest value of 0.419 detected in RM130 (Table 2 ). Indigenous rice varieties in NE India showed high level of genetic diversity with an overall allelic richness of 10.205 per locus and a gene diversity value of 0.776, while the agronomically improved varieties had significantly lower average allelic richness of 2.857 per locus and gene diversity was 0.459. The Riku Essays? A very high level of differentiation ( F ST = 0.754) was also detected among the rice varieties. The average observed number of alleles among indigenous rice varieties ranged from 1.14 ( Joha and Bherapawa ) to essaytown 3.29 ( Lallatoi ) while the corresponding value was only 1.00 for the agronomically improved varieties. Some of the dissertation abstracts elite traditional rice varieties (including Lallatoi , Mulahail , Aubalam , Mimutim ) showed very high levels of genetic diversity as measured in average numbers of alleles, rare alleles and Nei gene diversity. Two of those varieties exhibited relatively high numbers of essaytown coupon rare alleles ( Lallatoi = 4; Mimutim = 3).
Locus wise allele frequencies are presented in analysis Additional file 1 : Table S1. Nei’s gene diversity values ranged from 0.051 ( Bherapawa ) to 0.498 ( Lallatoi ) with an average of 0.223 across all indigenous varieties. Shannon information content varied widely across varieties from 0.072 ( Bherapawa ) to 0.854 ( Lallatoi ) and the average was 0.338 across varieties. The diversity parameters across varieties are presented in Table 1 . The pairwise genetic differentiation among varieties ( F ST ) ranged from essaytown, 0.375 to 1.000 and highly significant ( p 0.001). Stanford Mba Essay? The pairwise F ST values are given in Additional file 2 : Table S2. Population structure and F-statistics of different types of indigenous and agronomically improved rice varieties in essaytown NE India. Allelic richness is educational leadership, based on minimum sample size of 30 diploid individuals. H S(W) = average genetic diversity within type; F ST(W) = genetic differentiation within type. Coupon? Values in parenthesis represent standard deviation.
Analysis of molecular variance (AMOVA) based on 7 SSR loci of compare traditional and agronomically improved rice varieties in Northeast India. df, degree of coupon freedom; SS, sum of square; MS, Means of square. UPGMA tree based on chord genetic distance (Cavalli-Sforza and Edwards 1967 ) showing genetic relationships among 29 rice varieties in abstracts educational Northeast India. The UPGMA tree revealed that rice varieties clustered into smaller sub-groups based on coupon, type, grain qualities or geographic origin. For example, boro , jum , glutinous, and agronomically improved varieties clustered together into smaller sub-groups within Group-I ( indica ) while the Group-II ( japonica ) formed two sub-groups corresponding the geographic locations (Additional file 3 : Figure S1). A few sub-groups and varieties (marked with double asterisk), however, did not cluster with respective types or grain quality (Additional file 3 : Figure S1).
Principal component analysis of indigenous and structure essay agronomically improved rice varieties based on 7 SSR loci. Different varieties grouped together corresponding to two sub-species ( indica and essaytown japonica ). The relationship between ?K and K showing the highest peak at the riku essays, K = 2. Population structure of traditionally cultivated indigenous and agronomically improved rice varieties in the Eastern Himalayan region. The optimal value of K = 2. The present study revealed exceptionally high genetic variation, with an average allelic richness of 10.205 and an overall Nei’s gene diversity of 0.776 among indigenous rice varieties in NE India as compared to significantly low average allelic richness (2.798) and gene diversity (0.459) in agronomically improved types. Coupon? The levels of psychology genetic diversity were also variable across different varieties and essaytown much higher than the stanford analysis agronomically improved varieties (Table 1 ). Although the varieties represent only a sub-set of essaytown coupon total rice varieties in mfa creative writing the region, the gene diversity detected is higher than the coupon overall gene diversity of rice varieties reported from Yunnan province in social psychology China (0.706) (Tu et al. 2007 ) and Indonesia (0.68) (Thomson et al. 2007 ). The gene diversity detected in our study is comparable to essaytown coupon the overall gene diversity of wild rice O. rufipogon (0.77) and O. A Level? nivara (0.64) populations of the Vientiane Plain of Laos (Kuroda et al. 2007 ) and the gene diversity of O. Coupon? rufipogon in China (0.670) (Gao 2004 ). A previous study based on allozyme markers revealed a moderate genetic variability (Nei gene diversity = 0.341) among 289 rice varieties from NE India (Glaszmann et al. Mfa Creative? 1989 ). Essaytown Coupon? The higher gene diversity values detected in the present study could be attributable to high resolving power of mba essay microsatellite markers.
The present study revealed several indigenous rice varieties with high genetic diversity, which includes Lallatoi , Mulahail , Aubalam and Mimutim (Table 1 ). Despite the low yield, the traditional farmers in coupon Hailakandi area (Barak Valley region of Assam) have been cultivating Lallatoi , Mulahail and Aubalam for abstracts educational, over many generations presumably for its superior nutritional quality and better taste (personal communication). The local tribal group members in the Garo Hills of Meghalaya pointed out the superior agronomical qualities of Mimutim. Coupon? Our study revealed high genetic diversity in Mimutum , one of the highly valued rice varieties by native tribal groups. This reflects the importance of traditional knowledge in evaluation and conservation of indigenous crop genetic resources (Brush and the riku essays Meng 1998 ). Most of the indigenous rice varieties are maintained and cultivated by traditional farmers in narrow geographic regions.
However, traditional farming practices are in decline due to preference for agronomically improved varieties for higher yield. Therefore, appropriate conservation measures should be taken to promote the cultivation of indigenous varieties with local traditional knowledge. The genetic diversity maintained in a species is considered as a function of its ecological and evolutionary history (Hamrick and Godt 1996 ). The high genetic diversity among NE Indian rice varieties have been described in relation to morpho-physiological characters (Vairavan et al. 1973 ), enzymatic characters (Glaszmann et al. 1989 ), agro-morphological traits (Borkakati et al. Essaytown Coupon? 2000 ) and mba essay molecular markers including RAPD (Sarma and Bahar 2005 ) and ISSR (Bhuyan et al. Essaytown Coupon? 2007 ). The high genetic diversity among rice varieties in the NE Indian region could be attributable to combined effect of wide eco-geographical conditions, diverse agro-ecosystems associated with various rice farming practices and diverse human cultural preferences. High genetic diversity is also reported for the riku essays, other crop plants such as Zingiber officinale (Sajeev et al. 2011 ), Chilli (Yumnam et al. 2012 ), Curcuma species (Das et al. 2011 ), Citrus species (Hazarika 2012 ) commonly cultivated in NE India, highlighting the importance of the region for germplasm conservation of coupon many crop plants.
We compared the levels of genetic diversity among different types of rice cultivated in the riku essays NE India, and found that sali type possessed the highest gene diversity value of 0.747 and average allelic richness of 7.585. The majority of sali varieties are maintained by traditional farmers for specific traits such as aroma, grain size and shape, and tolerance to essaytown drought, insects and pests, which may contribute to the maintenance of high genetic variation. Jum type also showed high level of heterogeneity with gene diversity of 0.627 and essay compare contrast average allelic richness of 5.056. The traditional farming systems and local environment associated with adaptation to diverse conditions including water deficient habitats on the slopes of hilly regions may have contributed to the maintenance of high genetic variability among the jum type. Essaytown? Due to their inherent high genetic diversity, sali and jum types should be prioritized in conservation and management plans and structure compare contrast future breeding programs. The high F IS values among rice varieties of the coupon region could be due to predominantly selfing breeding system with a very low outcrossing in mfa creative writing O. Essaytown? sativa species (Oka 1988 ). The F ST results (Table 3 ) are also supported by AMOVA (Table 4 ) which indicated that 66% of the total variation was due to differentiation among varieties.
This indicates that rice varieties of the Eastern Himalayan region are highly differentiated. The UPGMA analyses using genetic distance data clustered rice varieties into two groups, which corresponded to stanford analysis O. sativa sub-species indica (Glaszmann and japonica 1987 ; Oka 1988 ; Khush 1997 ). Essaytown? These results agree with the previous isozyme data based finding that showed the occurrence of analysis two major groups of rice varieties in NE India (Glaszmann 1987 ). The PCA analysis and Model-based clustering method implemented in the STRUCTURE software also suggested the existence of two major groups corresponding to indica and japonica sub-species. The majority of varieties including agronomically improved rice varieties clustered as one group within the essaytown coupon sub-species indica . Most of the varieties were grouped into indica sub-species cluster while few varieties clustered into japonica sub-species. Vairavan et al. Writing? ( 1973 ) also reported similar results on the basis of amylose content, agronomic, and morphological characteristics. Our findings were similar to the study involving Indonesian landraces where 68% of the essaytown varieties were assigned as indica and the riku essays 32% as japonica (Thomson et al. Essaytown? 2007 ). However, a study of dissertation European rice collection revealed that 89% of the accessions belonged to japonica type (Courtois et al. 2012 ). The O. rufipogon showed intermediate position between indica and japonica types suggesting a possible common ancestry of coupon both indica and mfa creative japonica types. Although there was no clear differentiation among jum , sali , boro , and essaytown coupon glutinous varieties in the UPGMA and STRUCTURE analysis, the the riku essays PCA analysis separated the agronomically improved varieties into a distinct group (Figure 3 ) closely associated with the indica type.
This is expected as agronomically improved varieties included in the present study were derived from essaytown, indica type. Mba Essay? The STRUCTURE analysis did not show evidence for coupon, admixture between the indica and japonica types in a level essay writing almost all varieties. Essaytown Coupon? This could be attributable to predominantly selfing or autogamous nature of the breeding system and associated restricted gene flow among populations. Only a few varieties showed mixed ancestry of indica and japonica type (Figure 5 ), which may be either due to partial differentiation or rare introgression between the two types. Similar structuring reported among Asian cultivated rice Oryza sativa could be due to partial sharing of contrast their ancestral genetic polymorphism and/or recent gene flow (Gao and essaytown Innan 2008 ). Glaszmann et al. ( 1989 ) identified seven groups using isozyme markers and reported typical indica and japonica sub-species, suggesting that varieties mostly grown in mountainous areas of essay compare Meghalaya and Arunachal Pradesh belong to japonica . However, the present study revealed that varieties in the mountainous areas of Meghalaya and Arunachal Pradesh represent both japonica and indica types. Our results did not correspond to the five major groups described in Garris et al. ( 2005 ). In summary, high genetic diversity detected among traditional rice varieties in essaytown the Eastern Himalayan region of writing NE India is coupon, comparable to genetic diversity detected in wild rice populations in various parts of the world. Several varieties with high genetic diversity and cultural importance were found in stanford analysis Barak Valley region of Assam and Garo Hills of Meghalaya. The sali and jum type showed significantly higher levels of genetic diversity compared to coupon agronomically improved types. Dissertation? Rice varieties in NE India clustered into essaytown, two major groups corresponding to two sub-species, namely indica and japonica . The Riku Essays? Our findings highlights the importance of conservation of rice varieties in NE India as a means of preserving genetic diversity to maintain food security in the changing world.
Authors thank farmers in northeast India and International Rice Research Institute, Philippines for providing samples for the present study. Coupon? The study was financially supported by NSERC, FRQNT and Concordia University. We also gratefully acknowledge the uncw writing anonymous reviewers for their valuable comments which helped to improve the manuscript. Electronic supplementary material. The authors declare that they have no competing interests. BC, MLK and coupon SD contributed to the conceptual development of the project. BC carried out the molecular genetic work and data analyses guided by SD. BC, MLK and SD drafted the manuscript. All authors read and uncw mfa creative writing approved the final manuscript. Bhuyan N, Borah BK, Sarma RN: Genetic diversity analysis in traditional lowland rice ( Oryza sativa L.) of Assam using RAPD and ISSR markers. Coupon? Curr Sci 2007, 93: 967-972.
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